Isfahan University of Medical SciencesDental Research Journal1735-33279320120821Assessment of antifungal activity of ProRoot mineral trioxide aggregate and mineral trioxide aggregate-Angelus10811081ENAliKangarlouSamiraSofiabadiSaeedAsgaryFaranakMahjourOmidDianatDepartment of Endodontic, Iranian Center for Endodontic Research, Dental Research Center, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. omiddianat@gmail.comZahraYadegariFarnazYounessian20120821Background: Fungi may play a key part in periradicular diseases. The aim of this study was toevaluate and compare the antifungal properties of two root-end filling materials, ProRoot Mineraltrioxide aggregate (MTA) and MTA-Angelus, against Candida albicans using tube-dilution test.Materials and Methods: The antifungal properties of ProRoot MTA and MTA-Angelus againstC. albicans was assessed at 1, 24, and 48 hours following administration of two concentrationsof the antifungal agents (50 and 100 mg/ml). A total of 50 culture wells were divided into fourexperimental groups (Freshly mixed MTA, Freshly mixed MTA-Angelus, 24-h set MTA, and 24-hset MTA-Angelus) and two control groups. Each well was prepared for one specific agent with aspecific concentration. For the set groups, the mixture was prepared and left for 24 hours. Onemilliliter of suspension of fungal colonies with concentration of 104 CFU/ml was then added to themixtures in each well. All wells were incubated at 37°C and assessed at 1, 24, and 48 hours. Thisobservation was based on the turbidity of the suspension in the tubes. At each time point, 0.02 mlof each suspension was cultured on a Sabouraud dextrose agar plate to confirm C. albicans growth.The results were analyzed using Kruskal-Wallis test.Results: Although all fresh and set samples were incapable of killing C. albicans at 1 hour, theydemonstrated fungicidal ability on agar plates at 24 and 48-hour time points.Conclusion: MTA-angelus proved to be an effective antifungal agent compared to ProRoot MTAat concentrations of 50 mg/ml and 100 mg/ml.Key Words: Antifungal agents, Candida, endodontic