Socket preservation using demineralized freezed dried bone allograft with and without plasma rich in growth factor: A canine study

Abstract

Background: The accelerating effect of plasma rich in growth factors (PRGFs) in the healing of
extraction sockets has been demonstrated by some studies. The aim of the present study was to
histologically and histomorphometrically evaluate whether bone formation would increase by the
combined use of PRGF and demineralized freeze-dried bone allograft (DFDBA).
Materials and Methods: In four female dogs, the distal root of the second, third and fourth
lower premolars were extracted bilaterally and the mesial roots were preserved. The extraction
sockets were randomly divided into DFDBA + PRGF, DFDBA + saline or control groups. Two dogs
were sacrifi ced after 2 weeks and two dogs were sacrifi ced after 6 weeks. The extraction sockets
were evaluated from both histological and histomorphometrical aspects. The data were analyzed by
Mann-Whitney followed by Kruskal-Wallis tests using the Statistical Package for the Social Sciences
version 20 (SPSS Inc., Chicago, IL, USA). Signifi cant levels were set at 0.05.
Results: The least decrease in socket height was observed in the DFDBA + PRGF group
(0.73 ± 0.42 mm). The least decrease in the coronal portion was observed in the DFDBA + PRGF group
(1.38 ± 1.35 mm²). The least decrease in the middle surface was observed in the DFDBA group (0.61 ±
0.80 mm²). The least decrease in the apical portion was observed in the DFDBA group (0.34 ± 0.39 mm²).
Conclusion: The present study showed better socket preservation subsequent to the application of
DFDBA and PRGF combination in comparison with the two other groups. However, the difference
was not statistically signifi cant.
Key Words: Allograft, growth factor, guided bone regeneration, plasma rich, socket graft