In vitro evaluation of casein phosphopeptide-amorphous calcium phosphate effect on the shear bond strength of dental adhesives to enamel

Niloofar Shadman, Shahram Farzin Ebrahimi, Maryam Azizi Shoul, Hasti Sattari


Background: Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) is applied for

remineralization of early caries lesions or tooth sensitivity conditions and may affect subsequent

resin bonding. This in vitro study investigated the effect of CPP-ACP on the shear bond strength of

dental adhesives to enamel.

Materials and Methods: Sixty extracted human molar teeth were selected and randomly divided

into three groups and six subgroups. Buccal or lingual surfaces of teeth were prepared to create

a fl at enamel surface. Adhesives used were Tetric N-Bond, AdheSE and AdheSE One F. In three

subgroups, before applying adhesives, enamel surfaces were treated with Tooth Mousse CPP-ACP

for one hour, rinsed and stored in 37°C temperature with 100% humidity. This procedure was

repeated for 5 days and then adhesives were applied and Tetric N-Ceram composite was adhered

to the enamel. This procedure was also fulfi lled for the other three subgroups without CPP-ACP

treatment. After 24 hour water storage, samples were tested for shear bond strength test in a

universal testing machine. Failure modes were determined by stereomicroscope. Data were analyzed

by t-test and one-way analysis of variance with P < 0.05 as the level of signifi cance.

Results: In comparison between applied and non-applied CPP-ACP subgroups, there was no

signifi cant decrease in the shear bond strength to enamel only in Tetric N-Bond (P > 0.05). In nonapplied

CPP-ACP subgroups, there were statistically signifi cant differences among all subgroups.

Tetric N-Bond had the highest and AdheSE One F had the lowest shear bond strength.

Conclusion: CPP-ACP application reduces the shear bond strength of AdheSE and AdheSE One

F to enamel but not Tetric N-Bond.

Key Words: Casein phosphopeptide-amorphous calcium phosphate, enamel, etch-and-rinse,self-etch, shear bond strength

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