The effect of low-level helium-neon laser on oral wound healing

Abstract

Background: The effectiveness of low power lasers on incisional wound healing, because of
confl icting results of previous studies, is uncertain. Therefore, the aim of this study was to evaluate
the effects of low-level helium-neon (He-Ne) laser irradiation on wound healing in rat’s oral mucosa.
Materials and Methods: Sixty-four standardized incisions were carried out on the buccal mucosa
of 32 male Wistar divided into four groups of eight animals each. Each rat received two incisions
on the opposite sides of the buccal mucosa by a steel scalpel. On the right side (test side), a He-
Ne laser (632 nm) was employed on the incision for 40 s. Laser radiation was used just in 1st day,
1st and 2nd day, 1st and 3rd day, and continuous 3 days in groups of A, B, C, and D of rats, respectively.
The left side (control side) did not receive any laser. Histological processing and hematoxylin and
eosin staining were done on tissue samples after 5 days. Wilcoxon and Kruskal-Wallis tests were
used for statistical analysis.
Results: Histological analysis showed that the tissue healing after continuous 3 days on the laser
irradiated side was better than the control side, but there was no difference between the two
sides in each groups (P > 0.05).
Conclusion: This study showed that He-Ne laser had no benefi cial effects on incisional oral wound
healing particularly in 5 days after laser therapy. Future research in the fi eld of laser effects on oral
wound healing in human is recommended.

Key Words: Lasers, Helium, Histological Technique, Low-level laser therapies, oral, wound healings