Evaluation of the effects of two different bone resorption inhibitors on osteoclast numbers and activity: An animal study

Narges Naghsh, Sayed Mohammad Razavi, Mohsen Minaiyan, Mohammad Shahabooei, Reza Birang, Parichehr Behfarnia, Samira Hajisadeghi


Background: The aim of this study was to evaluate the effects of bone resorption inhibitors,
doxycycline (DOX) and erythromycin (EM), on osseous wound healing in rat alveolar socket.
Materials and Methods: In this randomized controlled trial, 45 8–10-week-old male Wistar rats
had their maxillary right molar extracted. They were divided into three groups of 15. In Group 1 normal saline, Group 2 DOX, and Group 3 EM were administered at the doses of 5 ml/kg/day,
5 mg/kg/day, and 2 mg/kg/day, respectively, for 7 consecutive days. The rats were sacrifi ced 7, 14, and
21 days after surgery. Real-time polymerase chain reaction was employed to evaluate the mRNA expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) and immunohistochemical staining for tartrate-resistant acid phosphatase (TRAP) to determine
osteoclasts. The data were analyzed by one-way analysis of variance followed by Tukey’s post hoc test using SPSS version 20. Signifi cant level was set at 0.05.
Results: The results showed that when drug-treated groups compared to control groups, RANKL gene expression signifi cantly decreased, TRAP + cells decreased on day 7. The RANKL/OPG ratios in the fi rst two weeks in the test groups were signifi cantly lower than the control group. There was no signifi cant difference in the studied indices between DOX and EM groups.
Conclusion: Following administration of DOX and EM, the number of osteoclasts and RANKL/
OPG ratio decreased suggesting their anti-osteoclastogenesis activity. These two drugs have no advantage over each other in increasing the bone formation.
Key Words: Immunohistochemistry, real-time polymerase chain reaction, tartrate-resistant acid phosphatase

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