Can tert‑butylhydroquinone improve the healing of extracted tooth socket in rats?

Somayeh Khoramian Tusi, Tahereh Eslam Manesh, Mahmood Sheikh Fathollahi, Ali Bagherian


Background: Tooth extraction causes an open wound in the soft and hard tissues. During the
inflammatory phase of the healing process, a large amount of free radicals are produced and cause
oxidative stress, which leads to tissue damage and delayed wound healing. Thus, in this study, we
evaluated the effect of tert‑butylhydroquinone (TBHQ), as an antioxidant, on the healing process
of tooth sockets in rats.
Materials and Methods: To conduct this experimental study, male Wistar rats (n = 42) were
divided into two groups. In each case, one upper second molar was extracted under general
anesthesia. After the extraction, the tooth sockets of the experimental group were treated with a
0.02% TBHQ solution (0.1 ml) while the same volume of distilled water placed in the sockets of
the control group. On days 3, 7, and 21 postoperatively, 7 rats from each group were euthanized,
and histological slides were prepared from their tooth sockets. The prepared slides were examined
histopathologically using a light microscope and compared using an independent two‑sample t‑test.
The significance level was set at 0.05.
Results: In the experimental group, a statistically significant (P = 0.003) increase in granulation tissue
was observed on day 3, in comparison to the control group. The extent of bony trabeculation was
also significantly higher in the TBHQ‑treated group than in the control group on day 21 (P < 0.001).
Conclusion: Considering the limitations of an experimental study, it can be concluded that TBHQ
may enhance the healing of the hard tissue in the tooth sockets.
Key Words: Antioxidants, rats, tooth extraction, wound healing

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