Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nanobioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

Batool Hashemi‑Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi


Background: The aim of this study was to compare the cytotoxicity and the biocompatibility of
three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous
dental pulp. Given the importance of scaffold and its features in tissue engineering, this study
demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano‑bioglass (nBG)
nanocomposite scaffold using electrospinning method.
Materials and Methods: This experimental study was conducted on normal exfoliated deciduous
incisors obtained from 6‑year‑old to 11‑year‑old healthy children. The dental pulp was extracted from
primary incisor teeth which are falling aseptically. After digesting the tissue with 4 mg/ml of type I
collagenase, the cells were cultured in medium solution. Identification of stem cells from human exfoliated
deciduous teeth was performed by flowcytometry using CD19, CD14, CD146, and CD90 markers.
Then, 1 × 104 stem cells were seeded on the scaffold with a diameter of 10 mm × 0.3 mm. Cell viability
was evaluated on days 3, 5, and 7 through methyl thiazol tetrazolium techniques (P < 0.05) on different
groups that they are groups included (1) PHB scaffold (G1), (2) PHB/chitosan scaffold (G2), (3) the
optimal PHB/chitosan/nBG scaffold (G3), (4) mineral trioxide aggregate (MTA), and (5) the G3 + MTA
scaffold (G3 + MTA). Data were analyzed with two‑way ANOVA at significance level of P < 0.05.
Results: The results indicated that the PHB/chitosan/nBG scaffold and PHB/chitosan/nBG
scaffold + MTA groups showed significant difference compared with the PHB/chitosan scaffold and
PHB scaffold groups on the 7th day (P < 0.05).
Conclusion: Thus, it can be concluded that the scaffold with nBG nanoparticles is more
biocompatible than the other scaffolds and can be considered as a suitable scaffold for growth and
proliferation of stem cells.
Key Words: Bioglass, chitosan, polyhydroxybutyrate, scaffold, stem cells

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