Email this article In vitro comparison of the effects of microwave irradiation and chemical and mechanical methods on the disinfection of complete dentures contaminated with Candida albicans
Abstract
Background: Dentures can be a source of infection or cross‑contamination with microorganisms
like Candida albicans. The aim of this in vitro study was to compare the effects of microwave irradiation,
chemical techniques, and a mechanical method (i.e., brushing) on the disinfection of complete
dentures contaminated with C. albicans.
Materials and Methods: In this experimental study, sixty sterilized dentures were divided into six
groups of 10 dentures each. The dentures in Groups 1 and 6 served as negative and positive controls,
respectively. The dentures (Groups 2–5) were contaminated with C. albicans and subjected to four
disinfection procedures: Corega tablets, 2% glutaraldehyde, brushing, and microwave irradiation.
Replicate aliquots (25 mL) of the suspension were cultured in Sabouraud dextrose broth. The
colonies were counted after 48 h of incubation at 37°C. To confirm long‑term disinfection, the
Trypticase soy broth (TSB) containers were stored at 37°C for 7 days, and turbidity was visually
observed. Data were analyzed with one‑way ANOVA and independent‑samples t‑test on SPSS the
level of statistical significance was set at 0.05.
Results: The dentures disinfected with microwave irradiation (650 W, 3 min) and glutaraldehyde (2%, 10 min)
exhibited no evidence of fungal growth after 48 h of incubation and also no turbidity in the TSB containers
after 7 days of incubation. However, the dentures disinfected using the mechanical method and Corega
tablets exhibited turbidity after 7 days and fungal growth after 48 h that was significantly more than that
in the two other methods (P = 0.000) and less than that in the positive control group (P = 0.000). The
differences between mechanical cleaning and cleansing tablet were not significant (P = 0.017).
Conclusion: Base on the results of this study, microwave irradiation (650 W, 3 min) and 2%
glutaraldehyde completely disinfected the dentures contaminated with C. albicans in the short
term and long term.
Key Words: Candida albicans, dentures, disinfection, glutaraldehyde, microwave
like Candida albicans. The aim of this in vitro study was to compare the effects of microwave irradiation,
chemical techniques, and a mechanical method (i.e., brushing) on the disinfection of complete
dentures contaminated with C. albicans.
Materials and Methods: In this experimental study, sixty sterilized dentures were divided into six
groups of 10 dentures each. The dentures in Groups 1 and 6 served as negative and positive controls,
respectively. The dentures (Groups 2–5) were contaminated with C. albicans and subjected to four
disinfection procedures: Corega tablets, 2% glutaraldehyde, brushing, and microwave irradiation.
Replicate aliquots (25 mL) of the suspension were cultured in Sabouraud dextrose broth. The
colonies were counted after 48 h of incubation at 37°C. To confirm long‑term disinfection, the
Trypticase soy broth (TSB) containers were stored at 37°C for 7 days, and turbidity was visually
observed. Data were analyzed with one‑way ANOVA and independent‑samples t‑test on SPSS the
level of statistical significance was set at 0.05.
Results: The dentures disinfected with microwave irradiation (650 W, 3 min) and glutaraldehyde (2%, 10 min)
exhibited no evidence of fungal growth after 48 h of incubation and also no turbidity in the TSB containers
after 7 days of incubation. However, the dentures disinfected using the mechanical method and Corega
tablets exhibited turbidity after 7 days and fungal growth after 48 h that was significantly more than that
in the two other methods (P = 0.000) and less than that in the positive control group (P = 0.000). The
differences between mechanical cleaning and cleansing tablet were not significant (P = 0.017).
Conclusion: Base on the results of this study, microwave irradiation (650 W, 3 min) and 2%
glutaraldehyde completely disinfected the dentures contaminated with C. albicans in the short
term and long term.
Key Words: Candida albicans, dentures, disinfection, glutaraldehyde, microwave
Refbacks
- There are currently no refbacks.