Effect of hemostatic agent on microshear bond strength of total‑etch and self‑etch adhesive systems

Maryam Hoorizad, Haleh Heshmat, Tara Ansar Hosseini, Seyedeh Sara Kazemi, Seyedeh Farnaz Tabatabaei


Background: Application of hemostatic agents can negatively affect the bond strength of adhesive
systems to dental substrate. This study aimed to assess the effect of ferric sulfate on microshear
bond strength of four total‑ and self‑etch adhesives to dentin after water storage.
Materials and Methods: In this in vitro study, 192 dentin slices with 2 mm thickness were made
of 64 extracted sound human third molars. The samples were divided into 8 groups (n = 24) as
follows: G1: Scotchbond Multi‑Purpose, G2: hemostatic agent + Scotchbond, G3: Adper Single
Bond, G4: hemostatic agent + Adper, G5: Clearfil SE Bond, G6: hemostatic agent + Clearfil, G7:
Single Bond Universal, and G8: hemostatic agent + Single Bond Universal. Composite cylinders with
0.7 mm diameter and 1 mm height were bonded to the surfaces. Each group was then divided into
two subgroups (n = 12) for water storage for 24 h and 3 months. The microshear bond strength
was then measured. Data were analyzed using the Shapiro–Wilk test, three‑way ANOVA, one‑way
ANOVA, and Tukey’s test (P < 0.05).
Results: Application of ferric sulfate decreased the bond strength of all bonding agents after both
24 h and 3 months of storage; but, this reduction was not statistically significant (P > 0.05). Single
Bond Universal at 24 h showed the highest and Adper Single Bond at 3 months showed the lowest
bond strength (P < 0.001).
Conclusion: Dentin contamination with hemostatic agents negatively affects the bond strength
of total‑ and self‑etch adhesives.
Key Words: Adhesives, hemostatics, shear strength

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