The effect of 10% alpha‑tocopherol solution and 5% grape seed extract on the microhardness and shear bond strength to bleached dentin

Abstract

Background: The aim of the study was to evaluate the effect of 10% alpha‑tocopherol and 5%
grape seed extract on the microhardness and shear bond strength (SBS) to bleached human dentin.
Materials and Methods: This in vitro study was done on 200 extracted premolars which were
decoronated and grinded to get flat dentin surface occlusaly. They were divided into four groups:
(a) bleaching, (b) bleaching and application of alpha‑tocopherol, (c) bleaching and application of
grape seed extract, and (d) control. Groups were further subdivided into Subgroups I and II (n = 30)
based on storage period before building with composite and were then tested for microhardness
and SBS determination. The data thus obtained was subjected to statistical analysis which was
performed using ANOVA test and post hoc Tukey’s test. The significance for the entire statistical
test was predetermined at P < 0.05.
Results: The results showed that the microhardness values were minimum in Group A (immediately
after bleaching) and maximum in control group. Comparison of data using one‑way ANOVA showed
that the P value was highly significant (P < 0.001) among the groups. The intergroup comparison
of SBS using post hoc Tukey’s tests revealed that the P value was significant (P < 0.05) when the
comparison was done between the Group A and Group C and Group B with Group D immediately
after bleaching.
Conclusion: Adverse effects of bleaching can be reversed with the application of 10%
alpha‑tocopherol and 5% grape seed extract over the dentinal surface microhardness and SBS.
Key Words: Alpha‑tocopherol, grape seed extract, hydrogen peroxide, tooth bleaching agents