Response of stem cells derived from human exfoliated deciduous teeth to Bio‑C Repair and Mineral Trioxide Aggregate Repair HP: Cytotoxicity and gene expression assessment

Abstract

Background: The aim of this study was to investigate and compare the cytotoxicity and gene
expression of Bio‑C Repair, Mineral Trioxide Aggregate (MTA) HP Repair, and Biodentine on stem
cells derived from exfoliated deciduous teeth.
Materials and Methods: In this in vitro study MTT assay was used to assess the cellular viability
at three different dilutions. The gene expression of Runt‑related transcription factor 2 (Runx2),
alkaline phosphatase (ALP), osteocalcin [OCN], and dentin matrix protein‑1 (DMP‑1) was measured
with real‑time polymerase chain reaction after 7 days, 14 days, and 21 days of incubation. One‑way
analysis of variance and Bonferroni posttest were used for statistical analysis (p=o.o5).
Results: After 72 h of incubation at dilution 1:4, stem cells derived from human exfoliated deciduous
teeth (SHEDs) cultivated in Biodentine, followed by Bio‑C Repair and MTA Repair HP reported
with highest cellular viability. The highest mRNA expression of Runx2, ALP, OCN, and DMP‑1 was
reported in SHEDs cultured in Biodentine (after 21 days of incubation).
Conclusion: Bio‑C Repair and MTA HP Repair are biocompatible and capable of odontogenic
differentiation similar to Biodentine when cultured in stem cells derived from exfoliated primary
teeth.
Key Words: Bio‑C Repair, cytotoxicity, gene expression, Mineral Trioxide Aggregate HP Repair, stem cells derived from human exfoliated deciduous teeth