Comparison of the number of bacterial colonies among four types of suture threads using simple loop method following periodontal surgery in patients with periodontitis: A single‑blind randomized clinical trial

Abstract

Background: This study investigated the number of bacterial colonies in four types of suture
threads, including silk, nylon, monocryl, and monocryl plus after periodontal surgery in patients
with moderate‑to‑severe periodontitis.
Materials and Methods: In this single‑blind randomized clinical trial, a total of 12 patients with
periodontitis who required periodontal flap surgery in all quadrants were included. One type of
suture, either silk, nylon, monocryl, or monocryl plus (coated with triclosan), was used following
each surgery in each quadrant. Sutures (3 mm) were removed from the mid, posterior, and anterior
regions of the flap 7 days postoperatively, and placed in a tube‑containing buffer medium to transfer
to the culture medium in a laboratory. Then, the bacterial colonies on each culture medium were
counted manually. Finally, the mean number of grown colonies (anaerobic and aerobic) was computed
and compared in each group of sutures. Data were analyzed by SPSS (Version 20) using the repeated
measures ANOVA and least significant difference follow‑up tests (α = 0.05).
Results: The findings of this study indicated a significantly higher mean number of aerobic, anaerobic,
and aerobic‑anaerobic colonies in silk suture than in the other three types of sutures (P < 0.05).
However, no significant difference was observed among other types of sutures (P > 0.05).
Conclusion: The results of this study showed that silk suture had a higher bacterial adhesion (aerobic,
anaerobic, and aerobic‑anaerobic) than monofilament sutures, including nylon, monocryl, and
monocryl plus. Moreover, no significant difference was found among the monofilament sutures in
the number of colonies grown on them.
Key Words: Colony forming unit, periodontal diseases, suture

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Samaneh Soltani : Pubmed,Google Scholar