Effect of active irrigation using shock wave‑enhanced emission photoacoustic streaming on dental pulp stem cell viability

Abstract

Background: Shock wave‑enhanced emission photoacoustic streaming (SWEEPS) is a novel
irrigation activation method based on photoacoustic streaming. The aim of this study was to look
into the impact of SWEEPS on the attachment and survival of dental pulp stem cells (DPSCs).
Materials and Methods: In this in vitro study, 34 standardized root segments were randomly
allocated into two groups: SWEEPS and the conventional conditioning group. After the irrigation,
human DPSCs were seeded on the internal walls of these samples, and the attachment and survival
of 30 of them were assessed on different days. The remaining two samples were observed using a
scanning electron microscope (SEM). Independent sample t‑test, Mann–Whitney U‑test, one‑way
ANOVA, Kruskal–Wallis, and two‑way ANOVA were used for data analysis with the level of
significance = 0.05.
Results: The viability of DPSCs was significantly greater in the SWEEPS group in comparison with
the conventional conditioning group (P = 0.029). Both groups have shown a significant increase in
the viability of DPSCs over time (P = 0.0001, P = 0.003). SEM results have shown a smear layer‑free
surface with firmly attached DPSCs in the SWEEPS group.
Conclusion: The results of this study indicated that active irrigation using SWEEPS could provide a superior surface in terms of viability and attachment of DPSCs compared to the conventional conditioning method.
Key Words: Lasers, mesenchymal stem cells, regenerative endodontics, root canal irrigants, smear layer

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Mehdi Vatanpour: Pubmed,Google Scholar