Comparative study of the pure AH Plus sealer and its combination with triple antibiotic paste at different concentrations on Enterococcus faecalis bacteria

Abstract

Background: In this study, the effects of pure AH Plus sealer and its combination with triple
antibiotic paste at different concentrations on Enterococcus faecalis bacteria have been investigated.
Materials and Methods: This in vitro study was accomplished by the means of a triple antibiotic
paste combination (minocycline, metronidazole, and ciprofloxacin) at different concentrations (0%,
1%, 5%, 10%, and 25%) with AH Plus sealer on E. faecalis bacteria. Sealers were set in an incubator for
1 h, 1 day, 3 days, and 7 days, and then 10 μL of bacteria solution was placed on all samples except
the negative control group. After drying for 1 h, 250 μL brain–heart infusion broth culture medium
was added, and it was cultured in solid media. Direct contact test technique was performed, and
the obtained data were analyzed by 1‑way ANOVA, 2‑way ANOVA, 3‑way ANOVA, and post hoc
test least significant difference. It should be noted that the data were evaluated at the significance
level of P < 0.05.
Results: The average of colony‑forming unit (CFU)/mL illustrated that there were no significant
differences between fresh antibiotic‑sealer combination, 1‑day set, and 3‑day set (P = 0.525), while
in sealer with 7‑day set, the average of CFU/mL was notably lower than other sets (P < 0.001).
The outcomes revealed a considerable variation by passing time and the number of CFU/mL was
remarkably reduced (P < 0.05). The data suggested that, by increasing the concentration, the average
of CFU/mL was decreased, whereas the average of CFU/mL did not have significant differences in
all concentrations of the antibiotic‑sealer combination compared to pure sealer (P < 0.05).
Conclusion: The concentration of 1% triple antibiotic in combination with root canal sealer may
become a crucial factor for inhibiting the growth of remaining bacteria.
Key Words: Antibiotic, Enterococcus faecalis, root canal sealer

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Maryam Zare Jahromi: Pubmed,Google Scholar