Combined effect of micro‑osteoperforation and vibration on interleukin‑1B, receptor activator of nuclear factor kappa‑B ligand, C‑C motif chemokine ligand 2, and tumor necrosis factor‑alpha in orthodontic patients: A parallel‑design randomized clinical tr

Abstract

Background: Bone remodeling is essential for orthodontic tooth movement. Techniques such as
micro‑osteoperforation (MOP) and vibration have been introduced to accelerate treatment by
stimulating biological responses.
Materials and Methods: Randomized clinical trial study adult orthodontic patients who
required bilateral extraction of maxillary first premolars were randomly assigned to two
groups (n = 10) of intervention and control. Both groups received MOP at the onset of
canine retraction. The intervention group also used a VPro5 vibrator for 28 days after the
onset of canine retraction in addition to MOP. GCF samples were collected before the onset
of orthodontic treatment (T0), right before canine retraction (T1), and after 24 h (T2),
7 days (T3), and 28 days (T4) by a paper point, and the GCF levels interleukin (IL)‑1 B, receptor
activator of nuclear factor kappa‑B ligand (RANKL), C‑C motif chemokine ligand (CCL) 2,
and tumor necrosis factor‑alpha (TNF)‑α were measured. Data were analyzed using SPSS v25.
Repeatedmeasures Analysis of Variance was employed to compare quantitative outcomes
between groups and over time, with statistical significance set at P < 0.05.
Results: The GCF level of the four inflammatory factors was not significantly different between the
two groups at any time point (P > 0.05). The trend of change in GCF level of the four inflammatory
factors was also the same in the two groups over time, such that the lowest level of all four markers
was recorded at T0. The highest level of TNF‑α was recorded at T2, and the highest level of RANKL,
IL1‑B, and CCL2 was recorded at T2 and T3.
Conclusion: It does not seem that combined MOP with vibration can increase the level of
inflammatory factors in GCF.
Key Words: Bone remodeling, inflammation mediators, physical stimulation, tooth movement techniques, vibration

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 Hannaneh Ghadirian: Pubmed,Google Scholar