Isfahan University of Medical SciencesDental Research Journal1735-33279120120218Comparative immunohistochemical analysis of angiogenesis and mast cell density in oral normal mucosa and squamous cell carcinoma888888ENGholamrezaJahanshahiDepartment of Oral and Maxillofacial Pathology and Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. jahanshahi@dnt.mui.ac.irMehdiSabaghian20120218Background: Squamous cell carcinoma (SCC) is the most common malignancy in oral cavity.Angiogenesis is essential for the development and progression of SCC. Recently, some studieshave reported that mast cells may play a role in tumor progression via promoting angiognesis.Since the results of various studies on the role of mast cells in tumor progression is not uniform,the aim of this study was to define the possible role of mast cells in the process of angiogenesisby determining the microvessel density (MVD) and mast cell density (MCD) and the associationbetween them in oral normal mucsoa and oral SCC.Materials and Methods: In this retrospective analytical study, paraffinized specimens from 22cases of normal mucosa and 20 cases of well-differentiated oral SCC were selected. Microvesselswere stained by using immunohistochemical technique with anti-CD34 antibody and mast cellswith toluidine blue and then were counted at 400× magnification in hot-spot areas under a lightmicroscope. The results were analyzed by using t- test and Pearson’s exams. P values less than 0.05was considered to be significant.Results: A significant correlation was noted between MVD and MCD in normal oral mucosa(P<0.001), but in spite of a higher density of mast cells and microvessels observed in oral SCCcompared to normal mucosa, there was no significant correlation between them (P=0.731).Conclusion: These findings showed that factors other than mast cells may play a role in theupregulation of tumor angiogenesis in oral SCC.Key Words: Angiogenesis, mast cell, oral squamous cell carcinoma