Isfahan University of Medical SciencesDental Research Journal1735-33279120120218Evaluating the levels of salivary alkaline and acid phosphatase activities as biochemical markers for periodontal disease: A case series894894ENSaritaDabraDepartment of Periodontology and Oral Implantology, Swami Devi Dyal Hospital and Dental College, Barwala, Distt. Panchkula, Haryana, India. saritadabra@yahoo.co.inPreetinderSingh20120218Background: The purpose of this study was to determine the salivary levels of alkaline phosphatase(ALP) and acid phosphatase (ACP) activities in patients with periodontal disease and to evaluatethe use of these enzymes as biochemical markers for periodontal tissue damage.Materials and Methods: In this prospective analytical study, we examined the activities of salivaryALP and ACP in patients with periodontal disease, before and after periodontal treatment. Theexperimental groups consisted of 20 gingivitis patients and 20 periodontitis patients and the controlgroup had healthy subjects (20 samples). The stimulated saliva of the patient was collected in asterile test tube and analyzed using Hitachi’s Diagnostic Automatic Analyser. Periodontal disease wasdetermined based on clinical parameters such as gingival index, probing depth and clinical attachmentloss. Patients with periodontal disease were under conventional periodontal treatment. The statisticalanalysis applied was Student’s t-test. Probabilities less than 0.05 (P < 0.05) were considered significant.Results: The obtained results showed statistically significant increased activities of ALP and ACPin saliva from patients with periodontal disease in relation to control group. A significant reductionin the enzyme levels was seen after conventional periodontal therapy.Conclusions: Based on these results, salivary ALP and ACP can be considered to be the biomarkersfor evaluating periodontal tissue damage.Key Words: Acid phosphatise, alkaline phosphatise, periodontal disease, saliva