Cytotoxicity evaluation of a copaiba oil-based root canal sealer compared to three commonly used sealers in endodontics

Abstract

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested

before their validation as fi lling materials. The aim of this study was to evaluate the cytotoxic effect

of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells.

Materials and Methods: The experimental groups were formed according to the culture medium

conditioned with the tested sealers, as follows: Control group (CG) (culture medium without

conditioning); Sealer 26 (S26) - culture medium + S26; Endofi ll (EF) - culture medium + EF; AH

Plus (AHP) - culture medium + AHP; and BS - culture medium + BS (Copaiba oil-based sealer). The

conditioned culture medium was placed in contact with 2 × 104 cells cultivated on 60 mm diameter

Petri dishes for 24 h. Then, hemocytometer count was performed to evaluate cellular viability,

using Trypan Blue assay. The normal distribution of data was tested by the Kolmogorov-Smirnov

test and the values obtained for cellular viability were statistically analyzed (1-way ANOVA, Tukey’s

test - P < 0.05), with a signifi cance level of 5%.

Results: S26, EF and AHP presented decreased cellular viability considerably, with statistical

signifi cance compared with CG (P < 0.05). BS maintained cellular viability similar to CG (P > 0.05).

Conclusion: The Copaiba oil-based root canal sealer presented promising results in terms of

cytotoxicity which indicated its usefulness as a root canal sealer.

Key Words: Copaiba oil-resin, copaifera genus, cytotoxicity, natural products, root canal sealer