Sponge amnion potential in post tooth extraction wound healing by interleukin‑6 and bone morphogenetic protein‑2 expression analysis: An animal study

Abstract

Background: Wound tooth extraction is a mechanical injury that traumatizes adjacent tissue.
Sponge amnion contains growth factors that can promote postextraction wound healing. Amnion
membranes can be transformed into sponge form rendering it easier to use. The aim of this
study is to analyze interleukin‑6 (IL‑6) and bone morphogenetic protein‑2 (BMP‑2) expression in
postextraction wound healing on the 1st and 7th day after sponge amnion application.
Materials and Methods: Twenty‑eight Wistar rats were used in this experimental descriptive
analytical study. Fourteen animals’ first right anterior mandible tooth was extracted; then, the
socket applied by sponge amnion and sutured (treatment group), while 14 others only sutured
(as control group). The alveolar bone tissue of animal was observed 1st and 7th days after extraction
and then was analyzed using immunohistostaining to identify the expression of IL‑6 and BMP‑2.
Statistical analysis was performed using one‑way ANOVA with the level of significance (P < 0.05).
Results: IL‑6 expression in the treatment group was significantly lower than the control group on
the 1st and 7th days (P = 0.000). BMP‑2 expression in the treatment group was significantly higher
than the control group on the 1st and 7th days (P = 0.000).
Conclusion: Sponge amnion can promote the healing process by increasing the expression of
BMP‑2 and decreasing IL‑6 expression.
Key Words: Amnion membrane, bone morphogenetic protein‑2, interleukin‑6, wound healing