Effect of local injection of injectable platelet‑rich fibrin on bone remodeling during orthodontic tooth movement in dogs

Abstract

Background: This study aimed to assess the effect of local injection of injectable platelet‑rich
fibrin (i‑PRF) on bone remodeling during orthodontic tooth movement in dogs.
Materials and Methods: In this animal study, the maxillary first premolars of four adult male
mixed‑breed dogs were bilaterally extracted, and a nickel–titanium closed coil spring with 150 g
force was placed between the canine and second premolar teeth. One quadrant of the maxilla
was randomly selected as the test quadrant, and 0.5 cc i‑PRF was injected into the periodontal
ligament (PDL) around the second premolar at 1, 21, and 42 days. The other quadrant served as
the control group and received saline injections. The dogs were sacrificed after 63 days, histological
sections were prepared, and changes in bone remodeling were assessed by comparing the percentage
of osteogenesis and number of osteoblasts and osteoclasts between the two groups by the Wilcoxon
and Mann–Whitney U‑tests (α = 0.05).
Results: The percentage of osteogenesis (16.0% ± 4.96% in i‑PRF and 13.5% ± 4.43% in the control),
the percentage of newly formed lamellar bone (10.25% ± 2.87% in i‑PRF and 8.75% ± 2.36% in the
control), the percentage of woven bone (5.75% ± 2.21% in i‑PRF and 4.75% ± 2.36% in the control),
the number of osteoblasts (15.0 ± 3.46 in i‑PRF and 11.75 ± 2.36 in the control), and the number
of osteoclasts (11.25 ± 4.34 in i‑PRF and 6.25 ± 2.62 in the control) were not significantly different
between the two groups (P > 0.05).
Conclusion: PDL injection of i‑PRF around the second premolars of dogs under orthodontic
force had no significant effect on bone remodeling.
Key Words: Bone remodeling, platelet‑rich fibrin, tooth movement techniques

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masoud feizbakhsh: Pubmed,Google Scholar